Tripartite combination of candidate pandemic mitigation agents: vitamin D, quercetin, and estradiol manifest properties of medicinal agents for targeted mitigation of the COVID-19 pandemic defined by genomics-guided tracing of SARS-CoV-2 targets in human cells
This conditional activation provides several advantages in which silent agonists can prime the 7nAChR, making it more responsive to endogenous ACh without causing desensitization typically seen with continuous agonist exposure
Relative gene expression was assessed by quantitative PCR (qPCR) on an ABI PRISM 7500 system (Applied Biosystems, Warrington, UK) using SYBR Green PCR Master Mix (Applied Biosystems)
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While their primary role is metabolism, CYP450 enzymes can generate ROS as byproducts during their catalytic cycle